smfish single molecule fluorescence in situ hybridization smfish probes Search Results


single-molecule fluorescence in situ hybridization (smfish)  (LGC Biosearch)
90
LGC Biosearch single-molecule fluorescence in situ hybridization (smfish)
Localization of amyB mRNAs in A. oryzae hyphae cultured in CD medium that contains glucose as the sole carbon source. <t>smFISH</t> was performed without (A) or with (B) the amyB probe. Scale bars, 10 μm. (C) Relative <t>fluorescence</t> intensity of amyB mRNAs at the apical and subapical regions of cultured cells in CD and CDmal media. Bars show mean with standard deviation. Significant difference at P < 0.05 among each lowercase (Tukey–Kramer test, n = 10).
Single Molecule Fluorescence In Situ Hybridization (Smfish), supplied by LGC Biosearch, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smfish+single+molecule+fluorescence+in+situ+hybridization+smfish+probes/single+molecule+fluorescence+in+situ+hybridization++smfish+/pmc07536267-42-0-22
Average 90 stars, based on 1 article reviews
single-molecule fluorescence in situ hybridization (smfish) - by Bioz Stars, 2026-10
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smfish single molecule fluorescence in situ hybridization smfish probes  (Biosearch Technologies Inc)
86
Biosearch Technologies Inc smfish single molecule fluorescence in situ hybridization smfish probes
Localization of amyB mRNAs in A. oryzae hyphae cultured in CD medium that contains glucose as the sole carbon source. <t>smFISH</t> was performed without (A) or with (B) the amyB probe. Scale bars, 10 μm. (C) Relative <t>fluorescence</t> intensity of amyB mRNAs at the apical and subapical regions of cultured cells in CD and CDmal media. Bars show mean with standard deviation. Significant difference at P < 0.05 among each lowercase (Tukey–Kramer test, n = 10).
Smfish Single Molecule Fluorescence In Situ Hybridization Smfish Probes, supplied by Biosearch Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smfish+single+molecule+fluorescence+in+situ+hybridization+smfish+probes/fluorescence+hybridization+in+molecule+probes+single+situ+smfish+smfish/pm34752731-479-46-71
Average 86 stars, based on 1 article reviews
smfish single molecule fluorescence in situ hybridization smfish probes - by Bioz Stars, 2026-10
86/100 stars
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single molecule rna fluorescence in situ hybridization smfish  (Cargill Inc)
86
Cargill Inc single molecule rna fluorescence in situ hybridization smfish
Localization of amyB mRNAs in A. oryzae hyphae cultured in CD medium that contains glucose as the sole carbon source. <t>smFISH</t> was performed without (A) or with (B) the amyB probe. Scale bars, 10 μm. (C) Relative <t>fluorescence</t> intensity of amyB mRNAs at the apical and subapical regions of cultured cells in CD and CDmal media. Bars show mean with standard deviation. Significant difference at P < 0.05 among each lowercase (Tukey–Kramer test, n = 10).
Single Molecule Rna Fluorescence In Situ Hybridization Smfish, supplied by Cargill Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smfish+single+molecule+fluorescence+in+situ+hybridization+smfish+probes/fluorescence+hybridization+in+molecule+rna+single+situ+smfish/pmc12712441-80-16-39
Average 86 stars, based on 1 article reviews
single molecule rna fluorescence in situ hybridization smfish - by Bioz Stars, 2026-10
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Image Search Results


Localization of amyB mRNAs in A. oryzae hyphae cultured in CD medium that contains glucose as the sole carbon source. smFISH was performed without (A) or with (B) the amyB probe. Scale bars, 10 μm. (C) Relative fluorescence intensity of amyB mRNAs at the apical and subapical regions of cultured cells in CD and CDmal media. Bars show mean with standard deviation. Significant difference at P < 0.05 among each lowercase (Tukey–Kramer test, n = 10).

Journal: Frontiers in Microbiology

Article Title: Single-Molecule FISH Reveals Subcellular Localization of α-Amylase and Actin mRNAs in the Filamentous Fungus Aspergillus oryzae

doi: 10.3389/fmicb.2020.578862

Figure Lengend Snippet: Localization of amyB mRNAs in A. oryzae hyphae cultured in CD medium that contains glucose as the sole carbon source. smFISH was performed without (A) or with (B) the amyB probe. Scale bars, 10 μm. (C) Relative fluorescence intensity of amyB mRNAs at the apical and subapical regions of cultured cells in CD and CDmal media. Bars show mean with standard deviation. Significant difference at P < 0.05 among each lowercase (Tukey–Kramer test, n = 10).

Article Snippet: Single-molecule fluorescence in situ hybridization (smFISH) analysis was performed essentially according to the manufacturer’s instructions with some modifications based on previous literature (LGC Biosearch Technologies; ; ).

Techniques: Cell Culture, Fluorescence, Standard Deviation

Localization of amyB and actA mRNAs in A. oryzae hyphae cultured in complete medium containing maltose. (A) smFISH was performed with amyB and actA probes added to an overnight culture in MPY. (B) Each image is a magnification of the region within the white rectangle in (A) . The white arrowhead indicates the nucleus located closed to the apex. (C) Line scan analyses of DAPI, amyB and actA mRNAs fluorescent signals were conducted through the hypha of (B) . The white arrowhead indicates the signal of the nucleus located closed to the apex. Note that the fluorescent signals of amyB mRNAs are not seen in the apical region, whereas those of actA mRNAs appeared more intense in the apical region. (D) Relative fluorescence intensity of amyB and actA mRNAs at apical and subapical regions. Bars show mean with standard deviation. **Statistically significant difference at P < 0.01 (Student’s t -test, n = 10). Scale bars, 20 μm (A) and 10 μm (B) .

Journal: Frontiers in Microbiology

Article Title: Single-Molecule FISH Reveals Subcellular Localization of α-Amylase and Actin mRNAs in the Filamentous Fungus Aspergillus oryzae

doi: 10.3389/fmicb.2020.578862

Figure Lengend Snippet: Localization of amyB and actA mRNAs in A. oryzae hyphae cultured in complete medium containing maltose. (A) smFISH was performed with amyB and actA probes added to an overnight culture in MPY. (B) Each image is a magnification of the region within the white rectangle in (A) . The white arrowhead indicates the nucleus located closed to the apex. (C) Line scan analyses of DAPI, amyB and actA mRNAs fluorescent signals were conducted through the hypha of (B) . The white arrowhead indicates the signal of the nucleus located closed to the apex. Note that the fluorescent signals of amyB mRNAs are not seen in the apical region, whereas those of actA mRNAs appeared more intense in the apical region. (D) Relative fluorescence intensity of amyB and actA mRNAs at apical and subapical regions. Bars show mean with standard deviation. **Statistically significant difference at P < 0.01 (Student’s t -test, n = 10). Scale bars, 20 μm (A) and 10 μm (B) .

Article Snippet: Single-molecule fluorescence in situ hybridization (smFISH) analysis was performed essentially according to the manufacturer’s instructions with some modifications based on previous literature (LGC Biosearch Technologies; ; ).

Techniques: Cell Culture, Fluorescence, Standard Deviation

Localization of amyB mRNAs in A. oryzae hyphae cultured in CDmal medium. smFISH was performed without (A,B) or with (C,D) the amyB probe. The images in (B,D) are magnifications of the regions within the white rectangles in (A,C) , respectively. (E) Line scan analysis of amyB fluorescent signals was conducted through the hypha shown in (D) . Yellow arrows in (D,E) depict corresponding regions. Note that the subapical areas show more intense fluorescent signals than the tip region. Scale bars, 20 μm (A,C) and 10 μm (B,D) .

Journal: Frontiers in Microbiology

Article Title: Single-Molecule FISH Reveals Subcellular Localization of α-Amylase and Actin mRNAs in the Filamentous Fungus Aspergillus oryzae

doi: 10.3389/fmicb.2020.578862

Figure Lengend Snippet: Localization of amyB mRNAs in A. oryzae hyphae cultured in CDmal medium. smFISH was performed without (A,B) or with (C,D) the amyB probe. The images in (B,D) are magnifications of the regions within the white rectangles in (A,C) , respectively. (E) Line scan analysis of amyB fluorescent signals was conducted through the hypha shown in (D) . Yellow arrows in (D,E) depict corresponding regions. Note that the subapical areas show more intense fluorescent signals than the tip region. Scale bars, 20 μm (A,C) and 10 μm (B,D) .

Article Snippet: Single-molecule fluorescence in situ hybridization (smFISH) analysis was performed essentially according to the manufacturer’s instructions with some modifications based on previous literature (LGC Biosearch Technologies; ; ).

Techniques: Cell Culture

Expression of amyB mRNA by shifting media from CD to CDmal. smFISH was performed with the amyB probe in cultures of CD before shifting (A) and after shifting to CDmal, at 30 min (B) , 60 min (C,D) , and 90 min (E–H) . Note that amyB fluorescent signals were observed through the hyphae (E,G) . The images in (D,F,H) are magnifications of the regions within the white rectangles in (C,E,G) , respectively. These enlarged images show amyB fluorescent signals in the apical (D) , intermediate (F) and basal regions (H) . Scale bars, 20 μm (A–C,E,G) and 10 μm (D,F,H) .

Journal: Frontiers in Microbiology

Article Title: Single-Molecule FISH Reveals Subcellular Localization of α-Amylase and Actin mRNAs in the Filamentous Fungus Aspergillus oryzae

doi: 10.3389/fmicb.2020.578862

Figure Lengend Snippet: Expression of amyB mRNA by shifting media from CD to CDmal. smFISH was performed with the amyB probe in cultures of CD before shifting (A) and after shifting to CDmal, at 30 min (B) , 60 min (C,D) , and 90 min (E–H) . Note that amyB fluorescent signals were observed through the hyphae (E,G) . The images in (D,F,H) are magnifications of the regions within the white rectangles in (C,E,G) , respectively. These enlarged images show amyB fluorescent signals in the apical (D) , intermediate (F) and basal regions (H) . Scale bars, 20 μm (A–C,E,G) and 10 μm (D,F,H) .

Article Snippet: Single-molecule fluorescence in situ hybridization (smFISH) analysis was performed essentially according to the manufacturer’s instructions with some modifications based on previous literature (LGC Biosearch Technologies; ; ).

Techniques: Expressing

Localization of amyB mRNA in A. oryzae hyphae cultured in complete media. A. oryzae hyphae were grown in GPY (A,B) or MPY (C,D) media. smFISH was performed without (A,C) or with (B,D) the amyB probe. The images in (E) are magnifications of the region within the white rectangle in (D) . The white arrowhead indicates the nucleus located closed to the apex. Note that the amyB fluorescent signals are not seen in the tip region. Scale bars, 20 μm (A–D) and 10 μm (E) .

Journal: Frontiers in Microbiology

Article Title: Single-Molecule FISH Reveals Subcellular Localization of α-Amylase and Actin mRNAs in the Filamentous Fungus Aspergillus oryzae

doi: 10.3389/fmicb.2020.578862

Figure Lengend Snippet: Localization of amyB mRNA in A. oryzae hyphae cultured in complete media. A. oryzae hyphae were grown in GPY (A,B) or MPY (C,D) media. smFISH was performed without (A,C) or with (B,D) the amyB probe. The images in (E) are magnifications of the region within the white rectangle in (D) . The white arrowhead indicates the nucleus located closed to the apex. Note that the amyB fluorescent signals are not seen in the tip region. Scale bars, 20 μm (A–D) and 10 μm (E) .

Article Snippet: Single-molecule fluorescence in situ hybridization (smFISH) analysis was performed essentially according to the manufacturer’s instructions with some modifications based on previous literature (LGC Biosearch Technologies; ; ).

Techniques: Cell Culture